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Mass spectrometry of glycoproteins [electronic resource] : methods and protocols / edited by Jennifer J. Kohler, Department o Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX, USA, Steven M. Patrie, Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA

Published
New York : Humana Press, [2013]
Physical Description
xi, 339 pages : illustrations (some color) ; 26 cm.
Additional Creators
Kohler, Jennifer, 1972- and Patrie, Steven M.
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Contents
Introduction to glycosylation and mass spectrometry / Steven M. Patrie, Michael J. Roth, and Jennifer J. Kohler -- PART I ENRICHMENT AND ISOLATION METHODS -- Tandem lectin weak affinity chromatography for glycoprotein enrichment / Zhi Yuan Ma, Yuliya Skorobogatko, and Keith Vosseller -- CSC technology: Selective labeling of glycoproteins by mild oxidation to phenotype cells / Andreas Hofmann, Damaris Bausch-Fluck, and Bernd Wollscheid -- Use of boronic acid nanoparticles in glycoprotein enrichment / Yawei Xu, Lijuan Zhang, and Haojie Lu -- Incorporation of unnatural sugars for the identification of glycoproteins / Balyn W. Zaro, Howard C. Hang, and Matthew R. Pratt -- Characterization of membrane-associated glycoproteins using lectin affinity chromatography and mass spectrometry / Yashu Liu, Jintang He, and David M. Lubman -- Sialic acid capture-and-release and LC-MS(n) analysis of glycopeptides / Jonas Nilsson and Göran Larson -- PART II SAMPLE PREPARATION -- In-solution digestion of glycoproteins for glycopeptide-based mass analysis / Eden P. Go, Kathryn R. Rebecchi, and Heather Desaire -- Nano-HPLC-MS of glycopeptides obtained after nonspecific proteolysis / Gerhild Zauner, Carolien A.M. Koeleman, André M. Deelder, and Manfred Wuhrer -- PART III SEPARATION METHODS -- Glycopeptide enrichment for MALDI-TOF mass spectrometry analysis by hydrophilic interaction liquid chromatography solid phase extraction (HILIC SPE) / Pia Hønnerup Jensen, Simon Mysling, Peter Højrup, and Ole Nørregaard Jensen -- Separation and identification of glycoforms by capillary electrophoresis with electrospray ionization mass spectrometric detection / Alina D. Zamfir, Corina Flangea, Alina Serb, Ana-Maria Zagrean, Andreas M. Rizzi, and Eugen Sisu -- Structural separations by ion mobility-MS for glycomics and glycoproteomics / Larissa S. Fenn and John A. McLean -- PART IV QUANTITATION METHODS -- Quantitative analysis of glycoprotein glycans / Ron Orlando -- Stable isotope labeling of N-glycosylated peptides by enzymatic deglycosylation for mass spectrometry-based glycoproteomics / Hiroyuki Kaji and Toshiaki Isobe -- Approaches for site mapping and quantification of o-linked glycopeptides / Peng Zhao, Stephanie H. Stalnaker, and Lance Wells -- Glycan profiling: Label-free analysis of glycoproteins / Yoshinao Wada -- PART V COMPUTATIONAL TOOLS -- Introduction to informatics in glycoprotein analysis / Kiyoko F. Aoki-Kinoshita -- Software tools for glycan profiling / Chuan-Yih Yu, Anoop Mayampurath, and Haixu Tang -- PART VI CASE STUDIES IN MASS SPECTROMETRY OF GLYCOPROTEINS -- Quantitative characterization of glycoproteins in neurodegenerative disorders using iTRAQ / Min Shi, Hyejin Hwang, and Jing Zhang -- Quantitative proteomic analysis of N-linked glycoproteins in human tear fluid / Lei Zhou and Roger W. Beuerman -- Elucidation of N-glycosites within human plasma glycoproteins for cancer biomarker discovery / Penelope Drake, Birgit Schilling, Brad Gibson, and Susan Fisher -- Characterizing the glycosylation state of therapeutic recombinant glycoproteins. / Nicole Samuels, David Kates, Jun Liu, and Joanne Severs.
Summary
This comprehensive new resource in the 'Methods in Molecular Biology' series features contributions from leading researchers who provide expert advice and reproducible, cutting-edge protocols for examining glycoproteins through mass spectrometry.--
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Genre(s)
ISBN
9781627031455 (alk. paper)
1627031456 (alk. paper)
9781627031462 (ebook)
Bibliography Note
Includes bibliographical references and index.
View MARC record | catkey: 10019886