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Guide to yeast genetics and molecular and cell biology. Part B [electronic resource] / edited by Christine Guthrie, Gerald R. Fink

Published:
Boston ; Amsterdam ; London : Academic Press, 2002.
Physical Description:
1 online resource (xxxvii, 623 pages) : illustrations
Additional Creators:
Fink, Gerald R. and Guthrie, Christine
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Contents:
Machine generated contents note: 1.Getting Started with Yeast / Fred Sherman -- 2.How to Set up a Yeast Laboratory / Cora Styles -- 3.Constructing Yeast Libraries / Haoping Liu -- 4.Transformation of Yeast by Lithium Acetate/Single-Stranded Carrier DNA/Polyethylene Glycol Method / R. Daniel Gietz / Robin A. Woods -- 5.Genetic Transformation of Saccharomyces cerevisiae Mitochondria / Nathalie Bonnefoy / Thomas D. Fox -- 6.LacZ Assays in Yeast / Steffen Rupp -- 7.Analysis of Budding Patterns / Matthew Lord / Tracy Chen / Atsushi Fujita / [et al.] -- 8.Uses and Abuses of HO Endonuclease / James E. Haber -- 9.Assays for Gene Silencing in Yeast / Fred van Leeuwen / Daniel E. Gottschling -- 10.Classical Mutagenesis Techniques / Christopher W. Lawrence -- 11.Introduction of Point Mutations into Cloned Genes / Brendan Cormack / Irene Castano -- 12.Insertional Mutagenesis: Transposon-Insertion Libraries as Mutagens in Yeast / Anuj Kumar / Susana Vidan / Michael Snyder -- 13.Tn7-Mediated Mutagenesis of Saccharomyces cerevisiae Genomic DNA in Vitro / Nurjana Bachman / Matthew C. Biery / Jef D. Boeke / [et al.] -- 14.Vector Systems for Heterologous Expression of Proteins in Saccharomyces cerevisiae / Martin Funk / Rainer Niedenthal / Dominik Mumberg / [et al.] -- 15.Cloning-Free Genome Alternations in Saccharomyces cerevisiae Using Adaptamer-Mediated PCR / Robert J. D. Reid / Michael Lisby / Rodney Rothstein -- 16.kar-Mediated Plasmid Transfer between Yeast Strains: Alternative to Traditional Transformation Methods / Bilyana Georgieva / Rodney Rothstein -- 17.Gene Disruption / Mark Johnston / Linda Riles / Johannes H. Hegemann -- 18.Synthetic Dosage Lethality / Vivien Measday / Philip Hieter -- 19.Saccharomyces Genome Database / Laurie Issel-Tarver / Karen R. Christie / Kara Dolinski / [et al.] -- 20.Three Yeast Proteome Databases: YPD, PombePd, and CalPD (MyoPathPD) / Csilla Csank / Maria C. Costanzo / Jodi Hirschman / [et al.] -- 21.Database Resources Relevant to Yeast Biology / Fran Lewitter -- 22.Searching Yeast Intron Data at Ares Lab Web Site / Leslie Grate / Manuel Ares, Jr. -- 23.Yeast Genomic Expression Studies Using DNA Microarrays / Audrey P. Gasch -- 24.Transcriptome Analysis of Saccharomyces cerevisiae Using Serial Analysis of Gene Expression / Munira A. Basrai / Philip Hieter -- 25.PCR-Based Engineering of Yeast Genome / Marie E. Petracek / Mark S. Longtine -- 26.ChIP-chip: A Genomic Approach for Identifying Transcription Factor Binding Sites / Christine E. Horak / Michael Snyder -- 27.Computational Approaches to Identifying Transcription Factor Binding Sites in Yeast Genome / Hao Li -- 28.Array-Based Methods for Identifying Protein-Protein and Protein-Nucleic Acid Interactions / Joseph F. Gera / Tony R. Hazbun / Stanley Fields -- 29.Building Protein-Protein Networks by Two-Hybrid Mating Strategy / Micheline Fromont-Racine / Jean-Christophe Rain / Pierre Legrain -- 30.Integrated Version of Reverse Two-Hybrid System for the Postproteomic Era / Hideki Endoh / Sylvie Vincent / Yves Jacob / [et al.] -- 31.Biochemical Genomics Approach to Map Activities to Genes / Eric M. Phizicky / Mark R. Martzen / Stephen M. McCraith / [et al.] -- 32.Use of Two-Dimensional Gels in Yeast Proteomics / Anders Blomberg.
Summary:
This volume and its companion, Volume 351, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include basic techniques, making mutants, genomics, and proteomics.
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ISBN:
0121822532 (electronic bk.)
9780121822538 (electronic bk.)
Note:
AVAILABLE ONLINE TO AUTHORIZED PSU USERS.
Bibliography Note:
Includes bibliographical references and indexes.
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