The zebrafish [electronic resource] : disease models and chemical screens / edited by H. William Detrich III, Monte Westerfield, Leonard I. Zon
- Published
- Amsterdam [Netherlands] ; London [England] : Elsevier/Academic Press, 2011.
- Edition
- 3rd ed.
- Physical Description
- 1 online resource : illustrations
- Additional Creators
- Detrich, H. William, Westerfield, Monte, and Zon, Leonard I.
Access Online
- ScienceDirect: ezaccess.libraries.psu.edu
- ScienceDirect: ezaccess.libraries.psu.edu
- Series
- Contents
- Machine generated contents note: pt. I Disease Models -- 1.Zebrafish Models of Germ Cell Tumor / James F. Amatruda -- I.Overview -- II.Germline Development -- III.Germ Cell Tumors -- IV.Methods for Studying Zebrafish Germ Cells -- Reference -- 2.Dissecting Mechanisms of Myelinated Axon Formation Using Zebrafish / David A. Lyons -- I.Introduction -- II.Methods and Materials -- III.Summary -- Acknowledgments -- References -- 3.In vivo Analysis of White Adipose Tissue in Zebrafish / John F. Rawls -- I.Introduction -- II.Rationale -- III.Materials -- IV.Methods -- V.Summary -- Acknowledgments -- References -- 4.Study of Host-Microbe Interactions in Zebrafish / Carol H. Kim -- I.Introduction -- II.Laboratory Protocols -- References -- 5.Hematopoietic Stem Cell Development: Using the Zebrafish to Identify the Signaling Networks and Physical Forces Regulating Hematopoiesis / Trista E. North -- I.Developmental Hematopoiesis -- II.Review of the Literature -- III.Use of Zebrafish to Investigate HSC Biology -- IV.Zebrafish Tools and Protocols -- References -- 6.Zebrafish as a Model for Hemorrhagic Stroke / Brant M. Weinstein -- I.Introduction and Basic Concepts -- II.Maintaining the Barrier Function of Blood Vessels -- III.Vascular Integrity and Stroke -- IV.Zebrafish as a Model for Studying Hemorrhage and Stroke -- V.Studying Vascular Integrity in the Zebrafish -- VI.Concluding Remarks -- References -- 7.A Zebrafish Model for VHL and Hypoxia Signaling / Fredericus van Eeden -- I.VHL Disease -- II.Chuvash Polycythemia -- III.VHL Regulates HIF Signaling -- IV.HIF-Independent Functions of pVHL -- V.VHL Animal Models -- VI.Zebrafish as a New Model for VHL -- VII.Loss of Vhl Leads to a Systemic Hypoxic Response in Zebrafish -- VIII.vhl Mutants Develop Chuvash Polycythemia and Blood Cell Maturation Defects -- IX.Zebrafish vhl Mutants Develop Angiogenesis Defects -- X.Zebrafish vhl Mutants Develop Pronephros Abnormalities -- XI.Translation from Fish to Humans -- XII.Concluding Remarks -- Acknowledgments -- References -- 8.Basement Membrane Diseases in Zebrafish / Matthias Hammerschmidt -- I.Introduction -- II.Basement Membrane-Related Zebrafish Pathologies -- III.Methodology for Zebrafish Studies of Basement Membrane Composition and Function -- IV.Summary -- Acknowledgments -- References -- 9.Zebrafish Provides a Novel Model for Lymphatic Vascular Research / Stefan Schulte-Merker -- I.Introduction -- II.Embryonic Lymphangiogenesis in Zebrafish -- III.Comparison Between Teleosts and Other Vertebrates -- IV.The Added Value of Zebrafish -- V.The Secondary Vessel System in Teleosts -- VI.Concluding Remarks -- VII.Methods -- Acknowledgments -- References -- 10.Not All Bones are Created Equal - Using Zebrafish and Other Teleost Species in Osteogenesis Research / P. Eckhard Witten -- I.Case Studies - Using Zebrafish for Addressing Biomedical Questions -- II.The Evolution of Skeletal Tissues -- III.Cartilage and Bone in Teleost Fish -- IV.Intermediate Skeletal Tissues -- V.Osteocyte-Containing Bone and Acellular Bone -- VI.Development of Teleost Vertebral Bodies, A Derived Process -- VII.Remodeling of the Teleost Skeleton -- VIII.Conclusions -- Acknowledgments -- References -- 11.Zebrafish Assays of Ciliopathies / Nicholas Katsanis -- I.Introduction -- II.Methods -- III.Zebrafish Ciliary Mutant Lines -- IV.General Considerations/Future Development -- Acknowledgment -- References -- 12.Infectious Disease Modeling and Innate Immune Function in Zebrafish Embryos / Annemarie H. Meijer -- I.Introduction -- II.Observation and Isolation of Innate Immune Cells -- III.Bacterial Infection Methods -- IV.Analysis of the Innate Immune Response -- V.Conclusions -- Acknowledgments -- References -- 13.Zebrafish as a Model for the Study of Human Cancer / A. Thomas Look -- I.Introduction: Zebrafish as a Cancer Model -- II.The Genetically Tractable Zebrafish -- III.Transgenic Models of Oncogenesis -- IV.Modeling the Loss of Tumor Suppression -- V.Modeling Tumor Cell Intravasation and Metastasis -- VI.Tumor-Initiating Cells -- VII.In vivo Small-Molecule Screens and Drug Discovery -- VIII.Conclusions -- References -- 14.Generating and Analyzing Fish Models of Melanoma / Manfred Schartl -- I.Introduction -- II.Transgenic Melanoma Lines in Zebrafish and Medaka -- III.Ultra-violet Irradiation Treatments -- IV.Basic Melanoma Pathology -- V.Histology -- VI.Molecular Analysis -- VII.RNA and Protein Isolation From Adult Fish Tumors -- VIII.Conclusions -- Acknowledgments -- References -- 15.Screening Pancreatic Oncogenes in Zebrafish Using the Gal4/UAS System / Steven D. Leach -- I.Introduction -- II.Transgenic Zebrafish with Gal4/UAS-Mediated eGFP-KRASG12V Expression in the Exocrine Pancreas -- III.Identification and Characterization of Pancreatic Tumors -- IV.Conclusions -- Acknowledgments -- References -- 16.Zebrafish Models of Rhabdomyosarcoma / David M. Langenau -- I.Introduction -- II.Rationale -- III.Material and Methods -- IV.Discussion -- Acknowledgments -- References -- 17.Transplantation in Zebrafish / Leonard I. Zon -- I.Introduction -- II.Rationale -- III.Methods -- IV.Discussion -- Acknowledgment -- References -- 18.Disease Modeling by Gene Targeting Using MicroRNAs / D.R. Love -- I.Introduction -- II.Mechanisms of MicroRNA Silencing -- III.Dicer and MicroRNAs in Zebrafish Development -- IV.Development of Vector-Based RNA Interference -- V.RNA Interference Work in Zebrafish -- VI.Use of siRNAs in the Zebrafish -- VII.Materials and Methods -- VIII.Results -- IX.Future Directions -- References -- 19.Fluorescent Imaging of Cancer in Zebrafish / David M. Langenau -- I.Introduction -- II.Fluorescent Proteins and Transgenic Models of Cancer -- III.Macroscopic Observation of Tumor Growth -- IV.Microscopic Observation in Tumorigenesis -- V.Confirming Transformation of Fluorescent-Labeled Tumor Cells by Cell Transplantation into Irradiated Recipient Animals -- VI.Identifying Tumor-Propagating Cell Subpopulations by Fluorescent Protein Expression and Cell Transplantation into Irradiated Recipient Animals -- VII.Use of Syngeneic Zebrafish for Cell Transplantation of Fluorescent-Labeled Tumors and Drug Discovery -- VIII.Cell Transplantation into Syngeneic Zebrafish to Accurately Assess Self-Renewal in Fluorescent-Labeled Cancer -- IX.Use of Syngeneic Zebrafish for Cell Transplantation: Single Cell Transplants -- X.Xenograft Transplantation of Fluorescently Labeled Cells into Zebrafish -- XI.Conclusions -- Acknowledgments -- References -- 20.The Role of Fanconi Anemia/BRCA Genes in Zebrafish Sex Determination / John H. Postlethwait -- I.Introduction -- II.Results and Discussion -- III.Summary -- Acknowledgments -- Acknowledgments -- pt. II Chemical Screens -- 21.Chemical Screening in Zebrafish for Novel Biological and Therapeutic Discovery / Leonard I. Zon -- I.Introduction -- II.Rationale -- III.Materials and Methods -- IV.Discussion/Caveats -- V.Summary -- Acknowledgments -- References -- 22.Using the Zebrafish Photomotor Response for Psychotropic Drug Screening / Randall T. Peterson -- I.Introduction -- II.The PMR Behavior -- III.Methods -- IV.Discussion -- Acknowledgments -- References -- 23.Designing Zebrafish Chemical Screens / Mark C. Fishman -- I.Rationale for Conducting Small Molecule Screens With Zebrafish -- II.Selection of Small Molecule Libraries -- III.Assay Design: Major Screen Types -- IV.Screening Methods -- V.Mechanism of Action Studies -- VI.Conclusions -- References.
- Summary
- This volume of Methods in Cell Biology is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This new volume on Disease Models and Chemical Screens, covers two rapidly emerging and compelling applications of the zebrafish. * Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls * This volume concentrates on Disease Models and Chemical Screens.
- Subject(s)
- Genre(s)
- ISBN
- 9780123813213 (electronic bk.)
0123813212 (electronic bk.)
9780123813206
0123813204 - Note
- AVAILABLE ONLINE TO AUTHORIZED PSU USERS.
- Bibliography Note
- Includes bibliographical references and index.
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