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Practical Liquid Chromatography [electronic resource] / by S. G. Perry, R. Amos, P. I. Brewer

Author
Perry, S. G.
Published
Boston, MA : Springer US, 1972.
Physical Description
online resource
Additional Creators
Amos, R., Brewer, P. I., and SpringerLink (Online service)
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Contents
1: Liquid Chromatography—The Background -- 1.1. Perspective -- 1.2. Plan of the Book -- 1.3. Chromatography and Its Basic Variants -- 1.4. Development Methods in Chromatography -- 1.5. Basic Experimental Arrangements -- 1.6. Measures of the Effectiveness of Chromatographic Separations -- 1.7. Retention Parameters -- References -- Further Reading -- 2: Band Spreading in Chromatography -- 2.1. Introduction -- 2.2. Factors Leading to Band Spreading -- 2.3. The “Random-Walk” Concept -- 2.4. Simple Mathematics of the Random Walk -- 2.5. Molecular Diffusion -- 2.6. Mass Transfer -- 2.7. Eddy Diffusion -- 2.8. Final Expression for Plate Height -- 2.9. Extrabed Contributions to Band Spreading -- 2.10. Columns versus Plates -- 2.11. Summary—Important Practical Consequences of Theory -- References -- Further Reading -- 3: Adsorption Chromatography: Mechanism and Materials -- 3.1. Introduction -- 3.2. Adsorption Isotherms -- 3.3. Nature of Adsorption Forces -- 3.4. Choice of Chromatographie System in LSAC -- 3.5. Variation of Adsorption Coefficient Kº with Solute Structure -- 3.6. Individual Adsorbents -- References -- Further Reading -- 4: Liquid Partition Chromatography—Mechanism and Materials -- 4.1. Introduction -- 4.2. Thermodynamics of Liquid-Liquid Partition -- 4.3. Selection of Phase Pairs -- 4.4. Support Materials -- 4.5. Further Practical Considerations -- References -- Further Reading -- 5: Exclusion Chromatography—Mechanism and Materials -- 5.1. Introduction -- 5.2. Peak Broadening in Exclusion Chromatography -- 5.3. Theories of Exclusion Chromatography -- 5.4. Stationary Phases for Exclusion Chromatography -- Appendix: Applications of Exclusion Chromatography -- References -- Further Reading -- 6: The Technique of Thin-Layer Chromatography -- 6.1. Introduction -- 6.2. Outline of Technique -- 6.3. The Stationary Phase -- 6.4. Preparation of TLC Plates -- 6.5. Application of the Sample -- 6.6. Choice of Mobile Phase -- 6.7. Development of Chromatogram -- 6.8. Reproducibility of RF Values -- 6.9. Detection of Solutes -- 6.10. Chromatographic Records -- 6.11. Direct Quantitative Analysis on the Layer -- 6.12. Removal of Solute from Adsorbent Layer -- 6.13. Use of Ancillary Techniques -- 6.14. Present Status and Future Outlook -- References -- Further Reading -- 7: Equipment for Column Chromatography -- 7.1. Introduction -- 7.2. Pneumatics -- 7.3. Gauges -- 7.4. Gradient Elution Supply -- 7.5. Sample Injectors -- 7.6. Columns and Fittings -- 7.7. Grinders and Sizing Equipment -- 7.8. Fraction Collectors -- 7.9. Detectors -- Appendix: A Short Guide to Commercial Equipment Suitable for Column Liquid Chromatography -- References -- Further Reading -- 8: Current Status of Liquid Chromatography: Place among Family of Chromatographic Techniques -- References.
Subject(s)
ISBN
9781468419351
Digital File Characteristics
text file PDF
Note
AVAILABLE ONLINE TO AUTHORIZED PSU USERS.
Part Of
Springer eBooks
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