- Light and electron microscopy studies of the nerve to the dorsal longitudinal flight muscle of Manduca sexta established the number of axons within nerve IIN1b and its branches. The neural lamella surrounding the main motor nerve contained small axons with neurosecretory inclusions. In the distal regions of the nerve near the muscle, the inclusions, or vesicles, were of two types: subspherical and irregular. Axons containing the larger "spherical" vesicles were found only in the proximal regions of the nerve.
Cobalt backfills of nerve IIN1b and the transverse nerve revealed that on both sides of the thorax there were at least two peripheral neurosecretory cells which were associated with the link and/or transverse nerves. One of these cells, neuron #1, contained large spherical electron-dense vesicles which appeared to be released into the haemolymph along its peripheral processes. The other cell, neuron #2, contained smaller subspherical vesicles.
The transverse nerve contained processes with vesicles similar to those of neuron #1. In addition, processes with tubulous and with irregular vesicles were observed. Some of these may originate in the median nervous system. The link nerve (peripheral nervous system) contained processes with tubulous, irregular and subspherical vesicles.
Light and electron microscopy CoCl(,2) backfills of nerve IIN1b produced intensification of neuron #2 as well as processes in the transverse nerve. Backfills of the transverse nerve produced intensified neuron #1 and processes in nerve IIN1b. Based on these studies a map of hypothetical and observed neurosecretory pathways between the median to transverse nerve, the two peripheral cell bodies, and peripheral nerves was made.
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- Dissertation Note:
- Ph.D. The Pennsylvania State University 1982.
- Source: Dissertation Abstracts International, Volume: 43-10, Section: B, page: 3099.
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