Actions for Three Dimensional Molecular Imaging for Lignocellulosic Materials [electronic resource].

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Three Dimensional Molecular Imaging for Lignocellulosic Materials [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy, 2011.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
Additional Creators
University of Notre Dame, United States. Department of Energy, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
The development of high efficiency, inexpensive processing protocols to render biomass components into fermentable substrates for the sequential processing of cell wall components into fuels and important feedstocks for the biorefinery of the future is a key goal of the national roadmap for renewable energy. Furthermore, the development of such protocols depends critically on detailed knowledge of the spatial and temporal infiltration of reagents designed to remove and separate the phenylpropenoid heteropolymer (lignin) from the processable sugar components sequestered in the rigid cell walls of plants. A detailed chemical and structural understanding of this pre-enzymatic processing in space and time was the focus of this program. We worked to develop new imaging strategies that produce real-time molecular speciation information in situ; extract sub-surface information about the effects of processing; and follow the spatial and temporal characteristics of the molecular species in the matrix and correlate this complex profile with saccharification. Spatially correlated SIMS and Raman imaging were used to provide high quality, high resolution subcellular images of Miscanthus cross sections. Furthermore, the combination of information from the mass spectrometry and Raman scattering allows specific chemical assignments of observed structures, difficult to assign from either imaging approach alone and lays the foundation for subsequent heterocorrelated imaging experiments targeted at more challenging biological systems, such as the interacting plant-microbe systems relevant to the rhizosphere.
Report Numbers
E 1.99:doe/er64497
doe/er64497
Subject(s)
Note
Published through SciTech Connect.
06/09/2011.
"doe/er64497"
Bohn, Paul W.; Sweedler, Jonathan V.
Type of Report and Period Covered Note
Final;
Funding Information
FG02-07ER64497
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