The morphologies of breast cancer cell lines in three-dimensionalassays correlate with their profiles of gene expression [electronic resource].

Published:: Washington, D.C. : United States. Dept. of Energy, 2007.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
Additional Creators:: United States. Department of Energy and United States. Department of Energy. Office of Scientific and Technical Information

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Summary:

3D cell cultures are rapidly becoming the method of choice for the physiologically relevant modeling of many aspects of non-malignant and malignant cell behavior ex vivo. Nevertheless, only a limited number of distinct cell types have been evaluated in this assay to date. Here we report the first large scale comparison of the transcriptional profiles and 3D cell culture phenotypes of a substantial panel of human breast cancer cell lines. Each cell line adopts a colony morphology of one of four main classes in 3D culture. These morphologies reflect, at least in part, the underlying gene expression profile and protein expression patterns of the cell lines, and distinct morphologies were also associated with tumor cell invasiveness and with cell lines originating from metastases. We further demonstrate that consistent differences in genes encoding signal transduction proteins emerge when even tumor cells are cultured in 3D microenvironments.

Report Numbers:

E 1.99:lbnl--62407
lbnl--62407

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Note:

Published through SciTech Connect.
01/31/2007.
"lbnl--62407"
": KP1104010"
Molecular Oncology 1 1 FT
Gray, Joe W.; Barcellos-Hoff, Mary Helen; Lee, Eva H.; Petersen, Ole W.; Myers, Connie A.; Kenny, Paraic A.; Spellman, Paul T.; Lee, Genee Y.; Bissell, MinaJ.; Semeiks, Jeremy R.; Neve, RichardM.; Lorenz, Katrin.
Ernest Orlando Lawrence Berkeley NationalLaboratory, Berkeley, CA (US)

Funding Information:

DE-AC02-05CH11231
443180

View MARC record | catkey: 13801855