DNA Microarray Technologies [electronic resource] : A Novel Approach to Geonomic Research
- Washington, D.C. : United States. Dept. of Energy. Office of Science, 2002.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
- Physical Description:
- 18-24 : digital, PDF file
- Additional Creators:
- United States. Department of Energy. Office of Science
United States. Department of Energy. Office of Scientific and Technical Information
- A cDNA microarray allows biologists to examine the expression of thousands of genes simultaneously. Researchers may analyze the complete transcriptional program of an organism in response to specific physiological or developmental conditions. By design, a cDNA microarray is an experiment with many variables and few controls. One question that inevitably arises when working with a cDNA microarray is data reproducibility. How easy is it to confirm mRNA expression patterns? In this paper, a case study involving the treatment of a murine macrophage RAW 264.7 cell line with tumor necrosis factor alpha (TNF) was used to obtain a rough estimate of data reproducibility. Two trials were examined and a list of genes displaying either a > 2-fold or > 4-fold increase in gene expression was compiled. Variations in signal mean ratios between the two slides were observed. We can assume that erring in reproducibility may be compensated by greater inductive levels of similar genes. Steps taken to obtain results included serum starvation of cells before treatment, tests of mRNA for quality/consistency, and data normalization.
- Published through SciTech Connect.
Journal of Undergraduate Research 2
Hinman, R.; Thrall, B.; Wong, K,.
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