Visualization of DNA Double-Strand Break Repair at the Single-Molecule Level [electronic resource].
- Washington, D.C. : United States. Dept. of Energy. Office of Science, 2003.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
- Physical Description:
- vp : digital, PDF file
- Additional Creators:
- Medical College of Georgia (1833-1873), United States. Department of Energy. Office of Science, and United States. Department of Energy. Office of Scientific and Technical Information
- Restrictions on Access:
- Free-to-read Unrestricted online access
- Exposure to low doses of ionizing radiation is universal. The signature injury from ionizing radiation exposure is induction of DNA double-strand breaks (DSBs). The first line of defense against DSBs is direct ligation of broken DNA ends via the nonhomologous end-joining pathway. Because even a relatively high environmental exposure induces only a few DSBs per cell, our current understanding of the response to this exposure is limited by the ability to measure DSB repair events reliably in situ at a single-molecule level. To address this need, we have taken advantage of biological amplification, measuring relocalization of proteins and detection of protein phosphorylation as a surrogate for detection of broken ends themselves. We describe the use of specific antibodies to investigate the kinetics and mechanism of repair of very small numbers of DSBs in human cells by the nonhomologous end-joining pathway.
- Report Numbers:
- E 1.99:826401
- Published through SciTech Connect.
225th American Chemical Society Meeting, New Orleans, LA (US), 03/23/2003--03/27/2003.
Takeda, Yoshihiko; Dynan, William S.; Li, Shuyi; Mernaugh, Raymond; Wragg, Stephanie.
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