Actions for Protection against radiation-induced mutations at the hprt locus by spermine and N,N{double_prime}-(dithiodi-2,1-ethanediyl)bis-1,3-propanediamine (WR-33278) [electronic resource].

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Protection against radiation-induced mutations at the hprt locus by spermine and N,N{double_prime}-(dithiodi-2,1-ethanediyl)bis-1,3-propanediamine (WR-33278) [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy. Office of Fissile Materials Disposition, 1993.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
Physical Description
23 pages : digital, PDF file
Additional Creators
Argonne National Laboratory, United States. Department of Energy. Office of Fissile Materials Disposition, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
The polyamine spermine and the disulfide NN″-(dithiodi-2,1-ethanediyl)bis-1,3-propanediamine (WR-33278) are structurally similar agents capable of binding to DNA. WR-33278 is the disulfide moiety of the clinically studied radioprotective agent (WR-2721). Because of their structural similarities, it was of interest to characterize and compare their radioprotective properties using the endpoints of cell survival and mutation induction at the hypoxanthine-guanine phosphoribosyl transferase (hprt) locus in Chinese hamster AA8 cells. In order to facilitate both the uptake of VM-33278 into cells and the direct comparison between the protective properties of WR-33278 and spermine, these agents were electroporated into cells. Electroporation alone reduced cell survival to 75% but had no effect on hprt mutation frequency. The electroporation of either spermine or WR-33278 at concentrations greater than 0.01 mM was extremely toxic. The exposure of cells to both electroporation and irradiation gave rise to enhanced cell killing and mutation induction. Cell survival values at a radiation dose of 750 cGy were enhanced by factors of 1.3 and 1.8 following electroporation of 0.01 mM of spermine and WR-33278, respectively, 30 min prior to irradiation. Neither agent was protective at a concentration of 0.001 mM. Protection against radiation-induced hprt mutations was observed for both spermine and WR-33278 under all experimental conditions tested.
Report Numbers
E 1.99:anl/cmb/pp--80243
anl/cmb/pp--80243
Subject(s)
Other Subject(s)
Note
Published through SciTech Connect.
06/01/1993.
"anl/cmb/pp--80243"
"DE93040881"
": Grant CA 37435"
Schwartz, J.L.; Grdina, D.J.; Shigematsu, N.
Type of Report and Period Covered Note
Topical; 06/01/1993 - 06/01/1993
Funding Information
W-31109-ENG-38
View MARC record | catkey: 13802794