Ecological Interactions Between Metals and Microbes [electronic resource].

Published:: Washington, D.C. : United States. Dept. of Energy. Office of Science, 2004.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
Additional Creators:: Purdue University, United States. Department of Energy. Office of Science, and United States. Department of Energy. Office of Scientific and Technical Information

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Summary:

Analyses of chromium resistant microbes. Culturable xylene-degrading and chromate-resistant microbes were obtained from chronically cocontaminated soil using a microcosm enrichment technique, and shown to correlate to dominant soil populations using culture independent techniques. The soil microbial community proved able to mount a respiratory response to addition of xylene in the presence of chromate. The majority of isolates belonged to the ubiquitous but poorly studied high %G+C Gram positive genus Arthrobacter, and exhibited considerable genotypic and phenotypic variability. Phenotypic assays uncovered a wide variation in the levels of chromate resistance, even between very closely related strains. Primers designed against conserved motifs in the known chrA chromate efflux gene failed to detect similar sequences among the chromate resistant Arthrobacter isolates obtained through enrichment.

Report Numbers:

E 1.99:nabir-1011901-2005
nabir-1011901-2005

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Published through SciTech Connect.
06/01/2004.
"nabir-1011901-2005"
Konopka, Allan E.

Funding Information:

NABIR 1011901

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