DNA sequencing by a single molecule detection of labeled nucleotides sequentially cleaved from a single strand of DNA [electronic resource].
- Washington, D.C. : United States. Dept. of Energy, 1993.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
- Physical Description:
- 6 pages : digital, PDF file
- Additional Creators:
- Los Alamos National Laboratory
United States. Department of Energy
United States. Department of Energy. Office of Scientific and Technical Information
- We are developing a laser-based technique for the rapid sequencing of large DNA fragments (several kb in size) at a rate of 100 to 1000 bases per second. Our approach relies on fluorescent labeling of the bases in a single fragment of DNA, attachment of this labeled DNA fragment to a support, movement of the supported DNA into a flowing sample stream, sequential cleavage of the end nucleotide from the DNA fragment with an exonuclease, and detection of the individual fluorescently labeled bases by laser-induced fluorescence.
- Published through SciTech Connect.
OE/LASE `93: International Society for Optical Engineering (SPIE) conference,Los Angeles, CA (United States),13-23 Jan 1993.
Martin, J.C.; Hammond, M.L.; Harding, J.D.; Ambrose, W.P.; Keller, R.A.; Goodwin, P.M.; Wilkerson, C.W.; Jett, J.H.; Marrone, B.L.; Schecker, J.A.; Shih, P.J.; Haces, A.
- Funding Information:
View MARC record | catkey: 13811861