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Computer assisted multiplex sequencing. Progress report, August 1, 1991--July 31, 1992 [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy, 1992.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
Physical Description
5 pages : digital, PDF file
Additional Creators
Harvard University, United States. Department of Energy, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
The objectives of this project are automation and optimization of multiplex sequencing. This year we have integrated direct transfer electrophoresis, automated multiplex hybridizations and automated film reading and applied this toward sequencing of three contiguous E. coli cosmids. Primers for the directed dideoxy sequence walking and sequence confirmation steps were synthesized with a 15 base tag complimentary to an alkaline phosphatase conjugate. A higher throughput synthesis device is well along in testing as are new automated hybridization devices. We have developed software for automatically annotating ORFs and databases of precise termini of proteis and RNA.
Report Numbers
E 1.99:doe/er/60565--6
doe/er/60565--6
Subject(s)
Other Subject(s)
Note
Published through SciTech Connect.
08/01/1992.
"doe/er/60565--6"
"DE93007144"
Church, G.M.
Type of Report and Period Covered Note
Annual; 01/01/1991 - 12/31/1992
Funding Information
FG02-87ER60565
View MARC record | catkey: 13811886