Microbial communities in subsurface environments [electronic resource] : Diversity, origin, and evolution. Project technical progress report, September 1, 1993--August 31, 1996
Published
Washington, D.C. : United States. Dept. of Energy, 1994. Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
This report summarizes the progress made from 9-1-93 to 5-1-94 on this DOE grant. As participants in the subsurface science program, the authors are assessing the influence of environmental conditions on the distribution and evolution of subsurface microorganisms employing molecular techniques. The approach utilizes 16S rRNA targeted oligonucleotide probes, polymerase chain reaction (PCR) amplification of gene sequences, and sequencing techniques. Continued progress towards identifying target sequences for selected microbial types and groups is being made by analysis of rRNA sequence data for subsurface microorganisms and other microorganisms in the rRNA databases. Hybridization probes for these target sequences are being produced and used to classify isolated strains of subsurface microbes into focus clades useful for testing origins hypotheses.