[Melting in adsorbed films]. Progress report 1992 [electronic resource].
- Washington, D.C. : United States. Dept. of Energy, 1992.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
- Physical Description:
- 2 pages : digital, PDF file
- Additional Creators:
- California Institute of Technology, United States. Department of Energy, and United States. Department of Energy. Office of Scientific and Technical Information
- Restrictions on Access:
- Free-to-read Unrestricted online access
- Over the past several years we have been developing a new approach to cloning large fragments of mammalian DNA in E. coli. which will permit detailed analysis of complex genomes. In January 1992 we began construction of an arrayed total human genomic library prepared in our BAC vector. Our goal is to create a 4-5X library which will be accessible for screening both by colony hybridization and by PCR. Our efforts in 1992 have been focused on expanding this library, characterizing specific clones isolated from the library, and demonstrating the use of BACs and Fosmids in creating physical maps. As a Model for the use of BACs in physical mapping, we have begun mapping human chromosome 22. In addition to their stability and ease of handling, BACs and Fosniids offer the advantage of permitting isolation of relatively large amounts of pure DNA which should greatly facilitate contig construction. We have created a 7X chromosome 22-specific Fosmid library consisting of clones obtained from DNA from a hybrid cell line.
- Report Numbers:
- E 1.99:doe/er/45192--t3
- Published through SciTech Connect.
- Type of Report and Period Covered Note:
- Annual; 12/31/1992 - 12/31/1992
- Funding Information:
View MARC record | catkey: 13812320