Actions for DM-CSF stimulated proliferation of rat alveolar macrophages and the effects of differing particulate burden levels in alveolar macrophages on the proliferative response [electronic resource].

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DM-CSF stimulated proliferation of rat alveolar macrophages and the effects of differing particulate burden levels in alveolar macrophages on the proliferative response [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy, 1993.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
Physical Description
22 pages : digital, PDF file
Additional Creators
Los Alamos National Laboratory, United States. Department of Energy, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
Particles are gradually redistributed among the lung`s population of alveolar macrophages (AM) at times well after they were originally deposited in the lung. One mechanism that may underlie this ``particle redistribution phenomenon`` is the in situ replication of particle-containing AM and the allocation of particles from dividing AM to daughter cells. Little is known about how the abilities of AM to proliferate may be affected by the containment of particles; conceivably, AM proliferation may be a particle burden-dependent process. In this study, we assessed the proliferative abilities of AM that contain differing burdens of phagocytized particles. Rats were intratracheally instilled with 2 × 10⁸ fluorescent, polystyrene microspheres (∼2 μM diam.), and several days thereafter their lungs were lavaged. The lavaged AM were analyzed using a multiparameter flow cytometer, and the AM were sorted according to their relative burdens of microspheres, i.e., low burdens, medium burdens, high burdens, based on their levels of fluorescence intensities. Control cells consisted of AM that were lavaged from the lungs of untreated animals and also passed through the flow cytometer. The sorted AM were then cultured in the presence of 0.3 ng/ml Granulocyte/Macrophage Colony-Stimulating Factor and cultured for up to 15 days. Control cells and the sorted AM all replicated similarly; no evidence was found to indicate that AM proliferation is either stimulated or decreased. Evidence was also obtained that indicated that when particle-containing AM divide, the particles they originally contained are distributed to their offspring.
Report Numbers
E 1.99:la-ur--93-1165
E 1.99: conf-9303155--1
conf-9303155--1
la-ur--93-1165
Subject(s)
Other Subject(s)
Note
Published through SciTech Connect.
05/01/1993.
"la-ur--93-1165"
" conf-9303155--1"
"DE93012629"
4. international inhalation symposium on toxic and carcinogenic effects of solid particles in the respiratory tract,Hanover (Germany),1-5 Mar 1993.
Lehnert, B.E.; Valdez, Y.E.; Pendergrass, D.
Funding Information
W-7405-ENG-36
View MARC record | catkey: 13812640