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Chromosome-specific cDNAs/STSs [electronic resource].

Published:
Washington, D.C. : United States. Dept. of Energy, 1991.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
Physical Description:
Pages: (7 pages) : digital, PDF file
Additional Creators:
Columbia University, United States. Department of Energy, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary:
This project has three main goals: (1) to construct high-quality normalized cDNA libraries from human tissues, (2) to develop methods for isolation of chromosome-specific cDNAs, (3) to contribute sequence information to the expanding cDNA/EST database. Our short term goal has been to construct a very high quality cDNA library from a 3 month post-natal total human brain. Several libraries were constructed and carefully characterized by DNA sequencing and restriction analysis while protocols were being streamlined. We have now generated a library which has the following main features: (1) very high complexity (10⁷ recombinants), (b) short poly (A) tails; (c) long size inserts (1 kb average); (d) undetectable co-cloning events; (e) background of non-recombinants. The next step will be to generate and test a normalized version of this brain cDNA library. Subsequently, single-stranded versions of the normalized library will be utilized for isolation of chromosome 13-specific cDNAs.
Report Numbers:
E 1.99:doe/er/61233-1
E 1.99: conf-9109321--1
E 1.99: conf-9110302--1
conf-9110302--1
conf-9109321--1
doe/er/61233-1
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Note:
Published through SciTech Connect.
11/01/1991.
"doe/er/61233-1"
" conf-9109321--1"
" conf-9110302--1"
"DE92004022"
3. genome sequencing conference; International symposium on identification of transcribed sequences, Hilton Head, SC (United States); Washington, DC (United States), 22-25 Sep 1991; 4-5 Oct 1991.
Soares, M.B.; Efstratiadis, A.
Funding Information:
FG02-91ER61233
View MARC record | catkey: 13812882