Microbial strain improvement for organosulfur removal from coal. [Rhodococcus rhodochrous] [electronic resource].
- Washington, D.C. : United States. Dept. of Energy, 1991.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
- Physical Description:
- Pages: (10 pages) : digital, PDF file
- Additional Creators:
- Institute of Gas Technology
United States. Department of Energy
United States. Department of Energy. Office of Scientific and Technical Information
- IGT has developed a microbial culture of Rhodococcus rhodochrous, designated as IGTS8, that is capable of specifically cleaving carbon-sulfur bonds in a range of organosulfur model compounds and is capable of removing organic sulfur from coal and petroleum without sacrificing the calorific value of the fuel. Although IGTS8 possesses the ability to specifically remove organic sulfur from coal, a major research need is to develop improved strains of microorganisms that possess higher levels of desulfurization activity and therefore will permit more favorable biodesulfurization process conditions: faster rates, more complete removal, and smaller reactor size. Strain improvement is the single most important aspect to the development of a practical coal desulfurization process. Microbial strain improvement can be accomplished using genetic engineering recombinant DNA techniques as well as by non-recombinant techniques of microbiology and genetics. Both approaches will be employed in the proposed research, with the objective of developing derivatives of IGTS8 with markedly enhanced levels of desulfurization activity and evaluating the ability of improved cultures to remove organic sulfur from coal. During the current quarter, desulfurization-deficient mutants of IGTS8 have been obtained and characterized, improved cloning vectors have been constructed, the construction and screening of geonomic libraries has been initiated, and a derivative of IGTS8 with enhanced desulfurization ability has been obtained.
- Published through SciTech Connect.
Kilbane, J.J. II.
Illinois Dept. of Energy and Natural Resources, Springfield, IL (United States)
- Funding Information:
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