Actions for [Physicochemical and microbiological factors influencing the bioavailability of organic contaminants in subsoils]. Progress report, [July 1, 1992--June 30, 1993] [electronic resource].

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[Physicochemical and microbiological factors influencing the bioavailability of organic contaminants in subsoils]. Progress report, [July 1, 1992--June 30, 1993] [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy, 1992.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
Physical Description
27 pages : digital, PDF file
Additional Creators
Michigan State University, United States. Department of Energy, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
We report progress in elucidating the microbiological variables important in determining the relative success of bacteria in utilizing soil-sorbed contaminants. Two bacterial species, Pseudomonas putida (ATCC 17484) and an Alcaligenes sp. isolated from petroleum contaminated soil are known to differ markedly in their ability to utilize soil-sorbed napthalene based on a kinetic comparison of their capability of naphthalene mineralization in soil-containing and soil-free systems. The kinetic analysis led us to conclude that strain 17484 had direct access to naphthalene present in a labile sorbed state which promoted the rapid desorption of naphthalene from the non-labile phase. Conversely, both the rate and extent of naphthalene mineralization by strain NP-Alk suggested that this organism had access only to naphthalene in solution. Desorption was thus limited and the efficiency of total naphthalene removal from these soil slurries was poor. These conclusions were based on the average activities of cells in soil slurries without regard for the disposition of the organisms with respect to the sorbent. Since both organisms degrade naphthalene by apparently identical biochemical pathways, have similar enzyme kinetic properties, and are both motile, gram negative organisms, we undertook a series of investigations to gain a better understanding of what microbiological properties were important in bioavailability.
Report Numbers
E 1.99:doe/er/60809--4
doe/er/60809--4
Subject(s)
Other Subject(s)
Note
Published through SciTech Connect.
12/31/1992.
"doe/er/60809--4"
"DE93007308"
Type of Report and Period Covered Note
Annual; 01/01/1992 - 12/31/1993
Funding Information
FG02-89ER60809
View MARC record | catkey: 13813677