Actions for PQQ [electronic resource] : Biosynthetic studies in Methylobacterium AM1 and Hyphomicrobium X using specific TC labeling and NMR. [Pyrroloquinoline quinones].

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PQQ [electronic resource] : Biosynthetic studies in Methylobacterium AM1 and Hyphomicrobium X using specific TC labeling and NMR. [Pyrroloquinoline quinones].

Published
Los Alamos, N.M. : Los Alamos National Laboratory, 1988.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
Physical Description
Pages: 10 : digital, PDF file
Additional Creators
Los Alamos National Laboratory and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
Using TC labeling and NMR spectroscopy we have determined biosynthetic precursors of pyrroloquinoline quinone (PQQ) in two closely related serine-type methylotrophs, Methylobacterium AM1 and Hyphomicrobium X. Analysis of the TC-labeling data revealed that PQQ is constructed from two amino acids: the portion containing N-6, C-7,8,9 and the two carboxylic acid groups, C-7' and 9', is derived-intact-from glutamate. The remaining portion is derived from tyrosine; the phenol side chain provides the six carbons of the ring containing the orthoquinone, whereas internal cyclization of the amino acid backbone forms the pyrrole-2-carboxylic acid moiety. This is analogous to the cyclization of dopaquinone to form dopachrome. Dopaquinone is a product of the oxidation of tyrosine (via dopa) in reactions catalyzed by monophenol monooxygenase (EC 1.14.18.1). Starting with tyrosine and glutamate, we will discuss possible biosynthetic routes to PQQ. 29 refs., 4 figs., 2 tabs.
Report Numbers
E 1.99:la-ur-88-3540
E 1.99: conf-8809195-1
conf-8809195-1
la-ur-88-3540
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Note
Published through SciTech Connect.
01/01/1988.
"la-ur-88-3540"
" conf-8809195-1"
"DE89002354"
1. international symposium on PQQ and quinoproteins, Delft, Netherlands, 5 Sep 1988.
Unkefer, C.J.; Duine, J.A.; Hanners, J.L.; Houck, D.R.; van Kleef, M.A.G.
Funding Information
W-7405-ENG-36
View MARC record | catkey: 13813805