Actions for Steps in the process of DNA binding and entry in transformation. [Pneumococcus] [electronic resource].

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Steps in the process of DNA binding and entry in transformation. [Pneumococcus] [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy, 1978.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
Physical Description
Pages: 13 : digital, PDF file
Additional Creators
Brookhaven National Laboratory, United States. Department of Energy, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
The DNA uptake phase of genetic transformation in S. pheumoniae is reviewed with emphasis on molecular interactions at each step. An initial, reversible binding appears to be dependent on the molecular concentration of donor DNA. Subsequent irreversible binding, limited to the numbr of molecules corresponding to a fixed number of receptor sites, requires potassium ions and energy. Competition of different DNAs for uptake occurs at the initial step, but depends on the size of the DNA as well as its molecular concentration. Single-strand breakage accompanies irreversible binding of DNA. The frequency of breaks does not appear to depend on DNA concentration. Entry of DNA follows irreversible binding. The entry step, in which donor DNA is converted to single strands, requires action of a membrane nuclease. In the membrane this nuclease is part ofa specific multiprotein structure, which may function as a unit in DNA binding and entry.
Report Numbers
E 1.99:bnl-24750
E 1.99: conf-780846-1
conf-780846-1
bnl-24750
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Note
Published through SciTech Connect.
01/01/1978.
"bnl-24750"
" conf-780846-1"
4. meeting on bacterial transformation and transfection, York, UK, 28 Aug 1978.
Lacks, S.
Funding Information
EY-76-C-02-0016
View MARC record | catkey: 13814019