(Genetics and biochemistry of surfactant synthesis in Rhodococcus sp. H13-A) [electronic resource].

Published:: Washington, D.C. : United States. Dept. of Energy, 1989.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
Physical Description:: Pages: (6 pages) : digital, PDF file
Additional Creators:: North Carolina State University, United States. Department of Energy, and United States. Department of Energy. Office of Scientific and Technical Information

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Summary:

The rationale for these studies resides is that biosurfactant synthesis occurs only when cells are grown with alkanes as sole source of carbon and energy. It is reasoned that biosurfactant synthesis is linked genetically to alkane oxidation and that the identification and characterization of the alk genes would provide information about the structural and regulator genes involved, in biosurfactant synthesis. Rhodococcus H13-A chromosomal DNA was isolated and digested with the restriction endonuclease Sau3A. The shuttle vector, pMVS301, was single site cleaved with Bgl II, phosphorylated, and the chromosomal DNA fragments agated into linearized pMVS301. The ligation mixture was used to transform competent E. coli HB101. The chromosomal DNA fragment has been cloned into pMVS301 which appears to contain genes encoding the initial oxidation of alkane. Electrophoration of Rhodococaus indicates transformation by this technique. Further studies are required to optimize conditions.

Report Numbers:

E 1.99:doe/er/13967-t1
doe/er/13967-t1

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Published through SciTech Connect.
01/01/1989.
"doe/er/13967-t1"
"DE93000913"

Funding Information:

FG05-88ER13967

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