Actions for Karyotype analysis of Chinese hamster chromosomes by flow microfluorometry [electronic resource].

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Karyotype analysis of Chinese hamster chromosomes by flow microfluorometry [electronic resource].

Published
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 1976.
Physical Description
Pages: 6 : digital, PDF file
Additional Creators
United States. Department of Energy. Office of Scientific and Technical Information
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Summary
Chromosomes from diploid and aneuploid Chinese hamster cells were isolated at neutral pH or following fixation in 50% acetic acid, stained with ethidium bromide, and analyzed by flow microfluorometry (FMF). In some experiments, the chromosomes were partially fractionated by zonal centrifugation on a sucrose gradient. FMF analysis produced a series of peaks and shoulders consistent with distributions expected on the basis of relative DNA content of the chromosomal components of these lines. Analysis of the fractions of partially purified chromosomes was consistent with visual microscopic determinations and demonstrated that specific chromosomes were associated with specific peaks in the FMF spectra. Partial separation of chromosomes according to size on sucrose gradients should facilitate the isolation of analyzable quantities of single chromosomes by electronic sorting. The spectra from euploid and aneuploid cells clearly demonstrate the usefulness of this method for rapid karyotype analysis from large numbers of cells. Subtle changes in chromosome morphology as revealed by G-band analysis can also be detected in flow systems.
Report Numbers
E 1.99:la-ur-76-459
E 1.99: conf-751158-1
conf-751158-1
la-ur-76-459
Subject(s)
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Note
Published through SciTech Connect.
01/01/1976.
"la-ur-76-459"
" conf-751158-1"
Workshop on automated cytogenetics, Pacific Grove, CA, USA, 30 Nov 1975.
Jett, J.H.; Deaven, L.L.; Stubblefield, E.
Los Alamos Scientific Lab., N.Mex. (USA)
Funding Information
W-7405-ENG-36
View MARC record | catkey: 13814127