THE LIFETIME OF BACTERIAL MESSENGER RNA [electronic resource].
- Washington, D.C. : U.S. Atomic Energy Commission, 1963. and Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
- Physical Description:
- 45 pages : digital, PDF file
- Additional Creators:
- U.S. Atomic Energy Commission and United States. Department of Energy. Office of Scientific and Technical Information
- Restrictions on Access:
- Free-to-read Unrestricted online access
- Puromycin, an inhibitor of protein synthesis, appears to act as an inhibitor at additional sites during the induction of β-galactosidase synthesis. No inhibition of the reactions proceeding during the first 20 seconds of induction was observed, but puromycin seems to prevent the accumulation of messenger RNA during the period between 20 seconds and the first appearance of enzyme activity after 3 minutes. When cells from a stationary culture are placed in fresh medium containing inducer for β-galactosidase, growth, as represented by increase in turbidity and by total protein synthesis, starts within 30 seconds. By contrast, β-galactosidase synthesis is greatly delayed compared with induction during exponential growth. Two other inducible enzymes show similar lags, but malic dehydrogenase, which requires no external inducer, shows no lag. The lags are not due to catabolite repression phenomena. They cannot be reduced by pretreatment of the culture with inducer, or by supplementing the fresh medium with amino acids or nucleotides. The lag is also demonstrated by an i⁻ mutant constitutive for β-galactosidase synthesis. An inhibitor of RNA synthesis, 6-azauracil, preferentially inhibits β-galactosidase synthesis compared with growth in both inducible and constitutive strains. It is suggested that these observations, together with many reports in the literature that inducible enzyme synthesis is more sensitive than total growth to some inhibitors and adverse growth conditions, can be explained by supposing that messenger RNA for normally inducible enzymes is biologically more labile than that for normally constitutive proteins. The implications of this hypothesis for the achievement of cell differentiation by genetic regulation of enzyme synthesis are briefly discussed.
- Published through SciTech Connect., 12/01/1963., "ucrl--11854", Journal of Molecular Biology 0 0 ISSN 0022-2836; JMOBAK FT, Calvin, M.; Moses, V., and Ernest Orlando Lawrence Berkeley NationalLaboratory, Berkeley, CA (US)
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