Screening for mutagenic response of six coded chemicals in the CHO/HGPRT system [electronic resource].
- Washington, D.C. : United States. Dept. of Energy, 1979.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
- Physical Description:
- Pages: 15 : digital, PDF file
- Additional Creators:
- Oak Ridge National Laboratory
United States. Department of Energy
United States. Environmental Protection Agency
United States. Department of Energy. Office of Scientific and Technical Information
- A set of stringent conditions required for quantifying mutation-induction to 6-thioguanine resistance, which selects for > 98% variants with altered hypoxanthine-guanine phosphoribosyl transferase (HGPRT) activity in a near-diploid Chinese hamster ovary (CHO) cell line have been defined. The system fulfills criteria for a specific locus mutational assay and is useful in studies of mechanisms of mammalian cell mutagenesis, determination of the mutagenicity of various physical and chemical agents, and screening for mutagenic activity of some industrial chemicals. Preliminary findings on the application of this system in screening for the mutagenic activity of 6 coded chemicals supplied by the International Program for the Evaluation of Short-Term Test for Carcinogenicity are presented. The study limits itself to concerns more with mutagenic response than quantitative mutagenesis. Although our limited study of 6 out of 42 chemicals in no way could be used for evaluating the performance of the CHO/HGPRT system; nevertheless, it could shed some light on identifying certain considerations necessary for applying this system for screening the mutagenic activity of unknown chemicals.
- Published through SciTech Connect.
International program for the evaluation of short-term tests for carcinogenicity, St Simons, GA, USA, 16 Oct 1979.
Hsie, A. W.; O'Neill, J. P.; Machanoff, R.; Schenley, R. L.; Brimer, P. A.
- Funding Information:
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