Arom enzyme conjugate of neurospora [electronic resource] : multiple proteases and subunit artifacts
- Washington, D.C. : United States. Dept. of Energy, 1978. and Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
- Physical Description:
- Pages: 18 : digital, PDF file
- Additional Creators:
- University of Tennessee, Knoxville, United States. Department of Energy, and United States. Department of Energy. Office of Scientific and Technical Information
- Restrictions on Access:
- Free-to-read Unrestricted online access
- The arom enzyme conjugate of Neurospora crassa is a multifunctional protein consisting of five enzymes which catalyze a sequennce of reactions leading to the biosynthesis of the aromatic amino acids. Previously, it was believed that this multienzyme system consisted of a set of physically associated but noncovalently linked enzymes. We now have good evidence that all five activities reside on a single polypeptide chain, and electron micrographs confirm that the enzyme conjugate is a dimer of this multifunctional polypeptide. The enzyme conjugate is very susceptible to proteolytic attack. However, a number of discrete proteolytic clips can occur before the system loses either its overall native structure or any one of its five activities. The uncontrolled action of a multiplicity of resident proteases in N. crassa during the extraction and purification of the conjugate led us to the false conclusion that this enzyme system consisted of noncovalently linked enzymes. Which of the many proteases that appear to be present in N. crassa are primarily involved in forming the artifactual subunits remains to be determined.
- Published through SciTech Connect., 01/01/1978., "conf-780433--1", Conference on limited proteolysis in microorganisms, Washington, DC, USA, 17 Apr 1978., and Allison, D.; Vitto, A.; Gaertner, F. H.; Cole, K. W.; Spady, G. E.
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