Actions for New apparatus for direct counting of. beta. particles from two-dimensional gels and an application to changes in protein synthesis due to cell density [electronic resource].

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New apparatus for direct counting of. beta. particles from two-dimensional gels and an application to changes in protein synthesis due to cell density [electronic resource].

Published
Los Alamos, N.M. : Los Alamos National Laboratory, 1987.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
Physical Description
Pages: 25 : digital, PDF file
Additional Creators
Los Alamos National Laboratory and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
A new method is described for scanning two-dimensional gels by the direct counting of ..beta.. particles instead of autoradiography. The methodology is described; results are compared with autoradiographic results; and data are presented demonstrating changed patterns of protein synthesis accompanying changes in cell density. The method is rapid and permits identification of differences in protein abundance of approximately 10% for a substantial fraction of the more prominent proteins. A modulation effect of more than 5 standard deviations, accompanying contact inhibition of cell growth, is shown to occur for an appreciable number of these proteins. The method promises to be applicable to a variety of biochemical and genetic experiments designed to delineate changes in protein synthesis accompanying changes in genome, molecular environment, history, and state of differentiation of the cell populations studied. 13 refs., 8 figs., 4 tabs.
Report Numbers
E 1.99:la-11029-ms
la-11029-ms
Subject(s)
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Note
Published through SciTech Connect.
07/01/1987.
"la-11029-ms"
"DE87012695"
Anderson, H.L.; Puck, T.T.; Shera, E.B.
Funding Information
W-7405-ENG-36
View MARC record | catkey: 13814545