Decreased uv mutagenesis in cdc8, a DNA replication mutant of Saccharomyces cerevisiae [electronic resource].
- Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 1978.
- Physical Description:
- Pages: 40 : digital, PDF file
- Additional Creators:
- United States. Department of Energy. Office of Scientific and Technical Information
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- Free-to-read Unrestricted online access
- A DNA replication mutant of yeast, cdc8, was found to decrease uv-induced reversion of lys2-1, arg4-17, tryl and ural. This effect was observed with all three alleles of cdc8 tested. Survival curves obtained following uv irradiation in cdc8 rad double mutants show that cdc8 is epistatic to rad6, as well as to rad1; cdc8 rad51 double mutants seem to be more sensitive than the single mutants. Since uv-induced reversion in cdc8 rad1 and cdc8 rad51 double mutants is like that of the cdc8 single mutants, we conclude that CDC8 plays a direct role in error-prone repair. To test whether CDC8 codes for a DNA polymerase, we have purified both DNA polymerase I and DNA polymerase II from cdc8 and CDC+ cells. The purified DNA polymerases from cdc8 were no more heat labile than those from CDC+, suggesting that CDC8 is not a structural gene for either enzyme.
- Published through SciTech Connect., 01/01/1978., "ur-3490-1570", Prakash, S.; Prakash, L.; Hinkle, D., and Rochester Univ., NY (USA)
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