Biological production of organic solvents from cellulosic wastes. Progress report, September 15, 1976--September 14, 1977 [electronic resource].
- Published
- Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 1977.
- Physical Description
- Pages: 42 : digital, PDF file
- Additional Creators
- United States. Department of Energy. Office of Scientific and Technical Information
Access Online
- Restrictions on Access
- Free-to-read Unrestricted online access
- Summary
- The objectives of this project are to optimize a modular process to convert cellulosic wastes to butanol and other oil-sparing chemicals. Research to date has focused on developing analytical methods, establishing a good data base and improving cellulase yields. Reliable assay methods for the Thermoactinomyces cellulase complex have been developed, measuring glucose and reducing sugar from filter paper and Avicel for total cellulase activity, viscosity change with carboxymethyl cellulose for the endoglucanase activity, and fluorescence change with methylumbelliferyl-..beta..-D-glucopyranoside for ..beta..-glucosidase activity. Isoelectric focusing within the range pH 3.5 to 6.0 has proved to be a quick and useful means of determining effective cellulase complex composition. About 10 different proteins are present in the fermentation broth. Detailed procedures for uv and near uv plus 8-methoxy-psoralen mutagenesis have been developed, and four mutants having 50% greater activity than the parent YX strain have been isolated. Cellulase production by Thermoactinomyces is growth related and is maximum when growth stops at 12 to 16 hours with 1 to 5% Avicel at pH 7.0 to 7.2 and 55/sup 0/C. A multistage fermenter has been assembled for optimization of butanol versus acetone production by Cl. acetobutylicum. A preliminary economic assessment, currently indicating butanol at just above 30 cents/lb, is being continuously updated.
- Report Numbers
- E 1.99:coo-4070-1
coo-4070-1 - Subject(s)
- Other Subject(s)
- Butanols
- Biosynthesis
- Cellulases
- Bioassay
- Biochemical Reaction Kinetics
- Cellulose
- Enzymatic Hydrolysis
- Fungi
- Mutations
- Acetone
- Acrylamide
- Ammonium Sulfates
- Cellobiose
- Chemical Reactors
- Clostridium
- Culture Media
- Design
- Enzymes
- Fermentation
- Glucose
- Organic Polymers
- Organic Solvents
- Ph Value
- Physical Properties
- Proteins
- Research Programs
- Substrates
- Alcohols
- Aldehydes
- Amides
- Ammonium Compounds
- Bacteria
- Bioconversion
- Biomass
- Carbohydrates
- Chemical Reactions
- Decomposition
- Disaccharides
- Energy Sources
- Hexoses
- Hydrolases
- Hydrolysis
- Hydroxy Compounds
- Ketones
- Kinetics
- Lysis
- Microorganisms
- Monosaccharides
- Oligosaccharides
- Organic Compounds
- Organic Nitrogen Compounds
- Oxygen Compounds
- Plants
- Polymers
- Polysaccharides
- Reaction Kinetics
- Renewable Energy Sources
- Saccharides
- Solvents
- Solvolysis
- Sulfates
- Sulfur Compounds
- Synthesis
- Note
- Published through SciTech Connect.
06/01/1977.
"coo-4070-1"
Humphrey, A.E.; Pye, E.K.; Forro, J.R.
Pennsylvania Univ., Philadelphia (USA) - Funding Information
- EY-76-S-02-4070
View MARC record | catkey: 13815033