Actions for Microbially-Mediated Subsurface Calcite Precipitation for Removal of Hazardous Divalent Cations [electronic resource].

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Microbially-Mediated Subsurface Calcite Precipitation for Removal of Hazardous Divalent Cations [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy. Office of Science, 2003.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
Physical Description
vp : digital, PDF file
Additional Creators
Idaho National Engineering and Environmental Laboratory, United States. Department of Energy. Office of Science, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
We are investigating microbially-mediated acceleration of calcite precipitation and co-precipitation of hazardous divalent cations (e.g., 90Sr) in calcite saturated subsurface systems. In theory, the addition of urea to an aquifer or vadose zone and its subsequent hydrolysis by indigenous microbes will cause an increase in alkalinity, pH and calcite precipitation. Lab studies indicated the ability of various bacteria to precipitate calcite through urea hydrolysis and that incorporation of strontium in biogenically-formed calcite is greater than in abiotically formed calcite. Results from a field experiment in a pristine location in the Snake River Plain aquifer involving the phased addition of molasses and then urea showed increases in total cell numbers, rate of urea hydrolysis and calcite formation during the study. The combined diagnostic approaches of microbiology, molecular ecology and analytical chemistry demonstrate the feasibility of this biogeochemical manipulation for subsurface remediation at arid Western DOE sites such as Hanford and INEEL.
Report Numbers
E 1.99:826394
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Note
Published through SciTech Connect.
03/27/2003.
225th American Chemical Society Meeting, New Orleans, LA (US), 03/23/2003--03/27/2003.
Smith, R.W.; Taylor, J.L.; Ingram, Jani C.; Colwell, Frederick S.; Ferris, F. Gratn; Reysenbach, A.-L.; Tyler, T.L.; Delwiche, M.E.; McLing, T.; Watwood, M.E.; Fujita, Yoshiko; Cortez, Marnie, M.; Banta, A.
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