Email RIS file
Bookmark
Report an Issue

Advanced experimental analysis of controls on microbial Fe(III) oxide reduction. First year progress report [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy. Office of Environmental Management, 1997.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
Physical Description
33 pages : digital, PDF file
Additional Creators
United States. Department of Energy. Office of Environmental Management and United States. Department of Energy. Office of Scientific and Technical Information
Access Online

Availability

I Want It
I Want It

Finding items...

Restrictions on Access
Free-to-read Unrestricted online access
Summary
'The authors have made considerable progress toward a number of project objectives during the first several months of activity on the project. An exhaustive analysis was made of the growth rate and biomass yield (both derived from measurements of cell protein production) of two representative strains of Fe(III)-reducing bacteria (Shewanellaalga strain BrY and Geobactermetallireducens) growing with different forms of Fe(III) as an electron acceptor. These two fundamentally different types of Fe(III)-reducing bacteria (FeRB) showed comparable rates of Fe(III) reduction, cell growth, and biomass yield during reduction of soluble Fe(III)-citrate and solid-phase amorphous hydrous ferric oxide (HFO). Intrinsic growth rates of the two FeRB were strongly influenced by whether a soluble or a solid-phase source of Fe(III) was provided: growth rates on soluble Fe(III) were 10--20 times higher than those on solid-phase Fe(III) oxide. Intrinsic FeRB growth rates were comparable during reduction of HF0 and a synthetic crystalline Fe(III) oxide (goethite). A distinct lag phase for protein production was observed during the first several days of incubation in solid-phase Fe(III) oxide medium, even though Fe(III) reduction proceeded without any lag. No such lag between protein production and Fe(III) reduction was observed during growth with soluble Fe(III). This result suggested that protein synthesis coupled to solid-phase Fe(III) oxide reduction in batch culture requires an initial investment of energy (generated by Fe(III) reduction), which is probably needed for synthesis of materials (e.g. extracellular polysaccharides) required for attachment of the cells to oxide surfaces. This phenomenon may have important implications for modeling the growth of FeRB in subsurface sedimentary environments, where attachment and continued adhesion to solid-phase materials will be required for maintenance of Fe(III) reduction activity. Despite considerable differences in the rate and pattern of FeRB growth with different Fe(III) forms, a roughly consistent long-term biomass yield of 5 to 15 mg protein per mmol Fe(III) reduced was observed during growth on different forms of Fe(III). These results should prove useful for quantitative modeling of FeRB growth and metabolism in various types of experimental and in situ anaerobic sedimentary systems.'
Report Numbers
E 1.99:emsp-55164--97
emsp-55164--97
Other Subject(s)
Note
Published through SciTech Connect.
07/01/1997.
"emsp-55164--97"
"DE00013477"
Roden, E.E.; Urrutia, M.M.
univ. of Alabama, Tuscaloosa, AL (US)
Type of Report and Period Covered Note
Annual; 12/31/1996 - 12/31/1997
View MARC record | catkey: 14138351