High-speed thermal cycling system and method of use [electronic resource].
- Berkeley, Calif. : Lawrence Berkeley National Laboratory, 1996.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
- Additional Creators:
- Lawrence Berkeley National Laboratory and United States. Department of Energy. Office of Scientific and Technical Information
- Restrictions on Access:
- Free-to-read Unrestricted online access
- A thermal cycling system and method of use are described. The thermal cycling system is based on the-circulation of temperature-controlled water directly to the underside of thin-walled polycarbonate microtiter plates. The water flow is selected from a manifold fed by pumps from heated reservoirs. The plate wells are loaded with typically 15-20 .mu.l of reagent mix for the PCR process. Heat transfer through the thin polycarbonate is sufficiently rapid that the contents reach thermal equilibrium with the water in less than 15 seconds. Complete PCR amplification runs of 40 three-step cycles have been performed in as little as 14.5 minutes, with the results showing substantially enhanced specificity compared to conventional technology requiring run times in excess of 100 minutes. The plate clamping station is designed to be amenable to robotic loading and unloading of the system. It includes a heated lid, thus eliminating the need for mineral oil overlay of the reactants. The present system includes three or more plate holder stations, fed from common reservoirs but operating with independent switching cycles. The system can be modularly expanded.
- Report Numbers:
- E 1.99:us 5508197
- Other Subject(s):
- High-Speed Thermal Cycling Method Thermal Cycling Method Described Thermal Cycling Based The-Circulation Temperature-Controlled Water Directly Underside Thin-Walled Polycarbonate Microtiter Plates Water Flow Selected Manifold Fed Pumps Heated Reservoirs Plate Loaded Typically 15-20 Reagent Mix Pcr Process Heat Transfer Polycarbonate Sufficiently Rapid Contents Reach Thermal Equilibrium Water 15 Complete Pcr Amplification Runs 40 Three-Step Cycles Performed 14 Minutes Results Substantially Enhanced Specificity Compared Conventional Technology Requiring Run Times Excess 100 Minutes Plate Clamping Station Designed Amenable Robotic Loading Unloading Heated Lid Eliminating Mineral Oil Overlay Reactants Plate Holder Stations Fed Common Reservoirs Operating Independent Switching Cycles Modularly Expanded Mineral Oil Substantially Enhanced Water Flow Thermal Cycling Thermal Cycling Thermal Cycling Heat Transfer Thermal Equilibrium Water Directly Microtiter Plate Titer Plate
- Published through SciTech Connect.
Hansen, Anthony D. A.; Jaklevic, Joseph M.
- Funding Information:
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