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Inductively coupled plasma mass spectrometry for stable isotope metabolic tracer studies of living systems [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy. Office of Energy Research, 1999.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
Physical Description
174 pages : digital, PDF file
Additional Creators
Ames Laboratory, United States. Department of Energy. Office of Energy Research, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
This dissertation focuses on the development of methods for stable isotope metabolic tracer studies in living systems using inductively coupled plasma single and dual quadrupole mass spectrometers. Sub-nanogram per gram levels of molybdenum (Mo) from human blood plasma are isolated by the use of anion exchange alumina microcolumns. Million-fold more concentrated spectral and matrix interferences such as sodium, chloride, sulfate, phosphate, etc. in the blood constituents are removed from the analyte. The recovery of Mo from the alumina column is 82 ± 5% (n = 5). Isotope dilution inductively coupled plasma mass spectrometry (ID-ICP-MS) is utilized for the quantitative ultra-trace concentration determination of Mo in bovine and human blood samples. The average Mo concentration in reference bovine serum determined by this method is 10.2 ± 0.4 ng/g, while the certified value is 11.5 ± 1.1 ng/g (95% confidence interval). The Mo concentration of one pool of human blood plasma from two healthy male donors is 0.5 ± 0.1 ng/g. The inductively coupled plasma twin quadrupole mass spectrometer (ICP-TQMS) is used to measure the carbon isotope ratio from non-volatile organic compounds and bio-organic molecules to assess the ability as an alternative analytical method to gas chromatography combustion isotope ratio mass spectrometry (GC-combustion-IRMS). Trytophan, myoglobin, and β-cyclodextrin are chosen for the study, initial observation of spectral interference of ¹³C⁺ with ¹²C¹H⁺ comes from the incomplete dissociation of myoglobin and/or β-cyclodextrin.
Report Numbers
E 1.99:is-t--1867
is-t--1867
Subject(s)
Other Subject(s)
Dissertation Note
Thesis (Ph.D.); PBD: 10 May 1999
Note
Published through SciTech Connect.
05/10/1999.
"is-t--1867"
"DE99003358"
Luong, E.
Funding Information
W-7405-ENG-82
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