Actions for Development of an efficient algal H{sub 2}-production system [electronic resource].

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Development of an efficient algal H{sub 2}-production system [electronic resource].

Published
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 1998.
Physical Description
pages 43-60 : digital, PDF file
Additional Creators
United States. Department of Energy. Office of Scientific and Technical Information
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Summary
Two major problems facing the development of a commercial photobiological algal H₂-producing system are the low rates of H₂ evolution and the sensitivity of the H₂-evolving enzyme system to O₂, a by-product of the photosynthetic water-splitting process. The objective of this project is to generate O₂-tolerant mutants from the green alga Chlamydomonas reinhardtii that are high producers of H₂ for use in a photobiological water-splitting, H₂-producing system that is cost effective, renewable, scalable, and non-polluting. The authors are currently employing a dual approach to address the O₂-sensitivity problem. The first approach, based on classical mutagenesis and selection procedures, depends on the ability of a mutagenized population of algal cells to survive under conditions that require them to either produce (H₂-production selection) or consume (photoreductive selection) H₂ in the presence of controlled amounts of O₂. The second approach, based on molecular genetic strategies, involves the cloning of the hydrogenase gene from C. reinhardtii and identification of expression factors required for optimal H₂-evolution activity. The latter approach will complement the first in the future goal of generating a commercial organism suitable for use in the private sector.
Report Numbers
E 1.99:nrel/cp--570-25315-vol.1
E 1.99: conf-980440--vol.1
conf-980440--vol.1
nrel/cp--570-25315-vol.1
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Note
Published through SciTech Connect.
08/01/1998.
"nrel/cp--570-25315-vol.1"
" conf-980440--vol.1"
"DE98007507"
US DOE hydrogen program technical review meeting, Alexandria, VA (United States), 28-30 Apr 1998.
Flynn, T.; Forestier, M.; Ghirardi, M.L.; Seibert, M.
National Renewable Energy Lab., Golden, CO (United States)
View MARC record | catkey: 14391197