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THE INHIBITION OF DEOXYRIBONUCLEASS I BY HYDEOXY-PIPHENYLS [electronic resource].

Published:
Washington, D.C. : U.S. Atomic Energy Commission, 1970.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
Physical Description:
57 pages : digital, PDF file
Additional Creators:
U.S. Atomic Energy Commission and United States. Department of Energy. Office of Scientific and Technical Information
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Summary:
DNA extracted with certain commercial brands of phenol is resistant to hydrolysis by the endonuclease DNAase I, while DNA extracted with other brands, or prepared by sodium chloride extraction, is susceptible to hydrolysis. The agent responsible for inhibition has been shown to be an oxidation product produced in some phenols. The inhibitor has been separated from other impurities in phenol by paper chromatography, and, by means of infrared and ultraviolet spectroscopy, it has been identified as o-hydroxybiphenyl. The kinetics of inhibition have been studied, and it was found that inhibition arises from direct action on the DNA rather than on the enzyme. Several hydroxybiphenyls and related compounds have been tested for inhibition, and a theory of molecular structure versus inhibitory effectiveness is suggested from this data. From studies on the chemical reversal of inhibition, as well as from ultraviolet spectral studies (in both absorption and circular dichroism), it appears that the mode of action of the inhibitors is hydrogen bonding to, and intercalation between, the basis of the nucleic acid.
Report Numbers:
E 1.99:ucrl--20108
ucrl--20108
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Note:
Published through SciTech Connect.
09/01/1970.
"ucrl--20108"
Calvin,M.; Adams, N.H.; Moses, V.; El-Badry, A.M.; Gottesfeld, J.M.
Ernest Orlando Lawrence Berkeley NationalLaboratory, Berkeley, CA (US)
Funding Information:
DE-AC02-05CH11231
View MARC record | catkey: 14391782