An axial approach to detection in capillary electrophoresis [electronic resource].
- Published
- Washington, D.C. : United States. Dept. of Energy, 1993.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy. - Physical Description
- 131 pages : digital, PDF file
- Additional Creators
- Ames Laboratory, United States. Department of Energy, and United States. Department of Energy. Office of Scientific and Technical Information
Access Online
- Restrictions on Access
- Free-to-read Unrestricted online access
- Summary
- Our approach involves on-axis illumination of the compounds inside the capillary detection region and is applied to absorbance and fluorescence detection. Absorbance measurements were made by focussing an incident laser beam into one capillary end; by using signals collected over the entire length of analyte band, this enhances the analytical path length of conventional absorbance detection 60x. This instrument offers a 15x improvement in detection limits. Three fluorescence detection experiments are discussed, all of which involve insertion of an optical fiber into capillary. The first uses a high refractive index liquid phase to obtain total internal reflectance along capillary axis, this reducing light scatter. The second uses a charge-coupled device camera for simultaneous imaging of a capillary array (this may be useful in genome sequencing, etc.). The third is a study of fluid motion inside the capillary under pressure-driven and electroosmotic flow. The thesis is divided into four parts. Figs, tabs.
- Report Numbers
- E 1.99:is-t--1656
is-t--1656 - Subject(s)
- Other Subject(s)
- Dissertation Note
- Thesis (Ph.D.); PBD: May 1993
- Note
- Published through SciTech Connect.
05/01/1993.
"is-t--1656"
"DE94005556"
Taylor, J.A. - Funding Information
- W-7405-ENG-82
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