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Conjugation of DNA to Silanized Colloidal Semiconductor Nanocrystalline Quantum Dots [electronic resource].

Published:
Bethesda, Md. : National Institutes of Health (U.S.), 2001.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
Physical Description:
vp : digital, PDF file
Additional Creators:
Lawrence Berkeley National Laboratory, National Institutes of Health (U.S.), and United States. Department of Energy. Office of Scientific and Technical Information
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Summary:
Water-soluble, highly fluorescent, silanized semiconductor nanocrystals with different surface charges were synthesized. To covalently attach the nanocrystals to biological macromolecules with a variety of mild coupling chemistries, the outermost siloxane shells were derivatized with thiol, amino, or carboxyl functional groups. Single- or double-stranded DNA was coupled to the nanocrystal surfaces by using commercially available bifunctional cross-linker. Conjugation had little effect on the optical properties of the nanocrystals, and the resulting conjugates were more stable than previously reported systems. By using the strategies developed in this study, most biomolecules can be covalently coupled to semiconductor nanocrystals. These nanocrystal-DNA conjugates promise to be a versatile tool for fluorescence imaging and probing of biological systems.
Report Numbers:
E 1.99:lbnl--50712
lbnl--50712
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Note:
Published through SciTech Connect.
09/10/2001.
"lbnl--50712"
"B& R 600305000"
Chemistry of Materials 14 5 ISSN 0897-4756; CMATEX FT
Bertozzi, Carolyn R.; Alivisatos, Paul.A.; Zanchet, Daniela; Bryant, Zev; Gerion, Daniele; Williams, Shara C.; Parak, Wolfgang J.; Micheel, Christine; Seitz, Markus; Bustamante, Carlos; Waerz, Anke S.; Bruehl, Richard E.
Funding Information:
AC03-76SF00098
80SH01
View MARC record | catkey: 14446939