An exploration of sequence specific DNA-duplex/pyrene interactions for intercalated and surface-associated pyrene species. Technical progress report [electronic resource].
- Washington, D.C. : United States. Dept. of Energy, 1994.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
- Physical Description:
- 15 pages : digital, PDF file
- Additional Creators:
- Georgia State University
United States. Department of Energy
United States. Department of Energy. Office of Scientific and Technical Information
- The use of both short (5-atom) and long (12-atom) covalent linking chains to attach, respectively, a pyrenesulfonate or a pyrenebutyrate moiety to a central region of a DNA duplex allows construction of DNA-duplex/pyrene assemblies of two types. Long linking chains permit pyrene to intercalate within the DNA duplex, while the short chains constrain pyrene to remain in the outer-surface region of the major-groove of the duplex. Electrochemical data suggest that reductive electron-transfer (ET) quenching of photoexcited pyrene (pyrene*) labels will be most exothermic for guanosine than for the other three DNA nucleosides and that oxidative ET quenching of pyrene* will be most exothermic for thymidine than for the other three DNA nucleosides. The study combines two effects, (1) differential DNA/pyrene geometries in covalent assemblies with different length linking chains and (2) differential ET quenching reactivities among the DNA nucleotides to explore sequence specific and duplex/pyrene association specific effects on DNA-base ionization reactions. This report describes progress in synthesizing target pyrene-labeled nucleosides and oligonucleotides, in commissioning our fluorescence lifetime measurement system, and in the photochemical behavior of pyrene-labeled nucleosides, single strands of DNA, and duplexes of DNA.
- Published through SciTech Connect.
- Type of Report and Period Covered Note:
- Annual; 01/01/1993 - 12/31/1994
- Funding Information:
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