Actions for UVA and visible light photons produce frank strand breaks in human P3 cell DNA as well as a new class of alkali-labile lesion [electronic resource].
UVA and visible light photons produce frank strand breaks in human P3 cell DNA as well as a new class of alkali-labile lesion [electronic resource].
- Published
- Washington, D.C. : United States. Dept. of Energy, 1994.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy. - Physical Description
- 6 pages : digital, PDF file
- Additional Creators
- Argonne National Laboratory, United States. Department of Energy, and United States. Department of Energy. Office of Scientific and Technical Information
Access Online
- Restrictions on Access
- Free-to-read Unrestricted online access
- Summary
- Human P3 epithelioid cells were exposed to UVA (290--400 nm), as well as to blue and green photons in the 400--520 mm region of the spectrum, and the production of strand breaks in their DNA was measured by using elution. After exposure to blue photons the profiles were convex (downturning), shown to be due to the induction of a mixture of frank single-strand DNA breaks (SSB) plus rapidly developing alkali-labile sites (ALS). as well as latent breaks that develop during the first 6 h of the elution, termed slowly developing alkali-labile sites (SDALS). A significant proportion of the lesions produced by P3 cells by blue light photons are SDALS, whereas green light photons at 520 nm and UVA photons at 365 nm produce no SDALS and the elution profiles are exponential. This is also the case for Chinese hamster ovary cells exposed to 405 nm, evidence that a unique chromophore in P3 cells that absorbs strongly in the blue region leads to the formation of SDALS. The similarity in induction of SDALS at 460 and 405 nm is evidence that the chromophore is not a porphyrin, because these compounds have sharp absorption maxima in the lower 400 nm region. The chemical nature of these lesions as well as the cellular responses to them remain enigmatic.
- Report Numbers
- E 1.99:anl/cmb/summ--82561
E 1.99: conf-9405134--1
conf-9405134--1
anl/cmb/summ--82561 - Subject(s)
- Other Subject(s)
- Note
- Published through SciTech Connect.
05/01/1994.
"anl/cmb/summ--82561"
" conf-9405134--1"
"DE94010745"
International symposiium on ozone, sun, cancer and molecular and cellular mechanisms and prevention,Paris (France),17-20 May 1994.
Peak, J.G.; Peak, M.J. - Funding Information
- W-31109-ENG-38
View MARC record | catkey: 14793460