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Molecular basis for the mutagenic and lethal effects of ultraviolet irradiation. Research accomplishments (1968 to present) [electronic resource].

Published
Baltimore, Md. : Johns Hopkins University, 1978.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy.
Physical Description
Pages: 13 : digital, PDF file
Additional Creators
Johns Hopkins University and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
Earlier work on the chemical basis of mutagenesis led to certain chemical generalities sufficient to explain how certain mutagens such as uv light and hydroxylamine functioned in information transfer systems (replicative, transcriptive and translational). When such modifications were applied to biologically active DNA in a controlled manner biological expression was non-stoichiometric because much of the damage was removed from the DNA by repair systems. Our efforts were then directed to these systems which led to: (1) the isolation, purification and characterization of endonucleases responsible for the first and controlling step in DNA repair - referred to as incision in both M. luteus and E. coli. The biological role of these enzymes was inferred in appropriate mutants; (2) the isolation, purification and characterization of exonucleases responsible for the removal or excision of damaged nucleotides in M. luteus and human placental trophoblasts; (3) the repair of uv damaged biologically active transforming and transfecting DNAs by purified endonucleases, exonucleases, DNA polymerase I and polynucleotide ligase from M. luteus and E. coli; (4) the characterization of the dual gene control for incision phenomenon in M. luteus and E. coli; and (5) isolation, purification and characterization of repair enzymes from human placenta (currently in progress).
Report Numbers
E 1.99:coo-2814-5
coo-2814-5
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Other Subject(s)
Note
Published through SciTech Connect.
01/01/1978.
"coo-2814-5"
Grossman, L.
Funding Information
EY-76-S-02-2814
View MARC record | catkey: 14794184