Actions for Gene-Based Detection of Microorganisms in Environmental Samples Using PCR

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Gene-Based Detection of Microorganisms in Environmental Samples Using PCR

Author
Roman, Monsi C.
Published
1997.
Physical Description
1 electronic document
Additional Creators
Paszko-Kolva, Christine, Lefkowitz, Elliot J., Taylor, Theresa B., Glass, John I., Cassell, Gail H., Wechser, Mark, and Albin, Michael
Online Version

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Unclassified, Unlimited, Publicly available.
Free-to-read Unrestricted online access
Summary
Contaminating microorganisms pose a serious potential risk to the crew's well being and water system integrity aboard the International Space Station (ISS). We are developing a gene-based microbial monitor that functions by replicating specific segments of DNA as much as 10(exp 12) x. Thus a single molecule of DNA can be replicated to detectable levels, and the kinetics of that molecule's accumulation can be used to determine the original concentration of specific microorganisms in a sample. Referred to as the polymerase chain reaction (PCR), this enzymatic amplification of specific segments of the DNA or RNA from contaminating microbes offers the promise of rapid, sensitive, quantitative detection and identification of bacteria, fungi, viruses, and parasites. We envision a small instrument capable of assaying an ISS water sample for 48 different microbes in a 24 hour period. We will report on both the developments in the chemistry necessary for the PCR assays to detect microbial contaminants in ISS water, and on progress towards the miniaturization and automation of the instrumentation.
Other Subject(s)
Collection
NASA Technical Reports Server (NTRS) Collection.
Note
Document ID: 19970027952.
Accession ID: 97N26826.
Rept-972424.
NASA-TM-112923.
NAS 1.15:112923.
International Conference on Environmental Systems (ICES); 14-17 Jul. 1997; Lake Tahoe, NV; United States.
Terms of Use and Reproduction
No Copyright.
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