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Fluorometric method for the determination of gas-phase hydrogen peroxide

Author
Kok, Gregory L.
Published
Dec 1, 1986.
Physical Description
1 electronic document
Additional Creators
Lazrus, Allan L.
Online Version

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Unclassified, Unlimited, Publicly available.
Free-to-read Unrestricted online access
Summary
The fluorometric gas-phase hydrogen peroxide procedure is based on the technique used by Lazrus et. al. for the determination of H2O2 in the liquid phase. The analytical method utilizes the reaction of H2O2 with horseradish peroxidase and p-hydroxphenylacetic acid (POPHA) to form the fluorescent dimer of POPHA. The analytical reaction responds stoichiometrically to both H2O2 and some organic hydroperoxides. To discriminate H2O2 from organic hydroperoxides, catalase is used to preferentially destroy H2O2. Using a dual-channel flow system the H2O2 concentration is determined by difference.
Other Subject(s)
Collection
NASA Technical Reports Server (NTRS) Collection.
Note
Document ID: 19870006108.
Accession ID: 87N15541.
NASA. Langley Research Center Future Directions for H sub x O sub y Detection; p 54-55.
Terms of Use and Reproduction
No Copyright.
View MARC record | catkey: 15700937