Preparation of guinea pig macrophage for electrophoretic experiments in space
- Published:
- May 1, 1979.
- Physical Description:
- 1 electronic document
Online Version
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- Unclassified, Unlimited, Publicly available.
Free-to-read Unrestricted online access - Summary:
- Methods of storage and cultivation of macrophage cells in preparation for space experiments were investigated. Results show that freezing and thawing immediately after extraction did not cause any change in viability or electrophoretic mobility of the cells. A prolonged storage at -80 C did cause cell damage as indicated by a 95% reduction in variable cells. Cell damage was decreased when Glycerol or Dimethyl Sulfoxide (DMSO) was added as a cryogenic protective agent. A 100% viability was observed in cultivation experiments after two weeks due to the additional serum. Results from gamma-glutamyl transpeptidase study showed a zero activity rate. It is suggested that a flat stationary field be used for the collection and use of macrophage. It was found that a 24-hour delay in obtaining macrophage cells helps to maintain a pure culture.
- Other Subject(s):
- Collection:
- NASA Technical Reports Server (NTRS) Collection.
- Note:
- Document ID: 19790019643.
Accession ID: 79N27814.
NASA-CR-158777. - Terms of Use and Reproduction:
- No Copyright.
View MARC record | catkey: 15725053