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Separation of granulocytes from whole blood by leukoadhesion, phase 1.

Published
Jun 1, 1976.
Physical Description
1 electronic document
Online Version

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Summary
Capillary glass tubes are investigated for the separation and retrieval of large quantities of viable granulocytes and monocytes from whole blood on a continuous basis from a single donor. This effort represented the feasibility demonstration of a three phase program for development of a capillary tube cell separation device. The activity included the analysis and parametric laboratory testing with subscale models required to design a prototype device. Capillary tubes 40 cm long with a nominal 0.030 cm internal diameter yielded the highest total process efficiency. Recovery efficiencies as high as 89% of the adhering cell population were obtained. Granulocyte phagocytosis of latex particles indicated approximately 90% viability. Monocytes recovered from the separation column retained their capability to stimulate human bone marrow colony growth, as demonstrated in an in vitro cell culture assay.
Other Subject(s)
Collection
NASA Technical Reports Server (NTRS) Collection.
Note
Document ID: 19760024806.
Accession ID: 76N31894.
MCR-76-272.
NASA-CR-147883.
Terms of Use and Reproduction
No Copyright.
View MARC record | catkey: 15734567