- Restrictions on Access:
- Open Access.
- Based on strong evidence for the essentiality of ribosome rescue in bacteria, I hypothesize that an alternative rescue factor exists in species where trans-translation is dispensable. I also hypothesize that there are conserved biochemical properties that all resolution mechanisms adhere to and may reflect a case of convergent evolution. In Section 2, I present secondary screening in Bacillus subtilis as part of an ongoing project to identify an alternative rescue factor using transposon-mutagenesis coupled to deep sequencing (Tn-seq). I extend discussion of the genetic properties of rescue factors to their shared inhibited activity on nonstop ribosomes with increasing 3-UTRs in Section 3. Taken together, the work in this masters thesis supports experiments to elucidate character and mechanism of ribosome rescue in bacteria.
- Dissertation Note:
- M.S. Pennsylvania State University 2017.
- Technical Details:
- The full text of the dissertation is available as an Adobe Acrobat .pdf file ; Adobe Acrobat Reader required to view the file.
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