Multi-Parametric Live Cell Microscopy of 3D Tissue Models [electronic resource] / edited by Ruslan I. Dmitriev
- Advances in Experimental Medicine and Biology, 0065-2598 ; 1035
- Current state-of-the-art 3D tissue models and their compatibility with live cell imaging -- Simultaneous Phosphorescence and Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC and Multi-Pulse Excitation -- Quantitative live cell FLIM imaging in three dimensions -- Three-dimensional tissue models and available probes for multi-parametric live cell microscopy: a brief overview -- Fabrication and handling of 3D scaffolds based on polymers and decellularized tissues -- Multi-parametric imaging of hypoxia and cell cycle in intestinal organoid culture -- Imaging of intracellular pH in tumor spheroids using genetically encoded sensor SypHer2 -- Application of fluorescence lifetime imaging (FLIM) to measure intracellular environments in a single cell -- Quantitative imaging of Ca2+ by 3D-FLIM in live tissues -- Live cell imaging of viscosity in 3D tumour cell models -- Live imaging of cell invasion using a multicellular spheroid model and lightsheet microscopy -- Raman imaging microscopy for quantitative analysis of biological samples.
- This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.
- Digital File Characteristics:
- text file PDF
View MARC record | catkey: 21837075