Actions for Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking [electronic resource].

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Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking [electronic resource].

Published
Washington, D.C. : United States. National Nuclear Security Administration, 2017.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description
Article numbers e0,180,503 : digital, PDF file
Additional Creators
United States. National Nuclear Security Administration, National Institutes of Health (U.S.), and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. Furthermore, a population of yeast cells of approximately 125,000 members is activated to roughly 55 Bq/cell by 18F uptake. An in vitro particle tracking experiment is performed with nearly 20 of these cells after decay to 32 Bq/cell. These cells are successfully identified and tracked simultaneously in this experiment. Our work extends the applicability of PEPT as a cell tracking method by allowing a number of cells to be tracked together, and demonstrating tracking for very low activity tracers.
Report Numbers
E 1.99:1393145
Subject(s)
Note
Published through SciTech Connect.
07/06/2017.
PLoS ONE 12 7 ISSN 1932-6203 AM
Seth T. Langford; Cody S. Wiggins; Roque Santos; Melinda Hauser; Jeffrey M. Becker; Arthur E. Ruggles; Chin-Tu Chen.
Univ. of Tennessee, Knoxville, TN (United States)
Funding Information
NA0001983
GM112496
View MARC record | catkey: 23492830