Simultaneous electrical recording of cardiac electrophysiology and contraction on chip [electronic resource].
- Washington, D.C. : United States. Dept. of Energy, 2017.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
- Physical Description:
- pages 1,732-1,739 : digital, PDF file
- Additional Creators:
- Lawrence Livermore National Laboratory
United States. Department of Energy
United States. Department of Energy. Office of Scientific and Technical Information
- Prevailing commercialized cardiac platforms for in vitro drug development utilize planar microelectrode arrays to map action potentials, or impedance sensing to record contraction in real time, but cannot record both functions on the same chip with high spatial resolution. We report a novel cardiac platform that can record cardiac tissue adhesion, electrophysiology, and contractility on the same chip. The platform integrates two independent yet interpenetrating sensor arrays: a microelectrode array for field potential readouts and an interdigitated electrode array for impedance readouts. Together, these arrays provide real-time, non-invasive data acquisition of both cardiac electrophysiology and contractility under physiological conditions and under drug stimuli. Furthermore, we cultured human induced pluripotent stem cell-derived cardiomyocytes as a model system, and used to validate the platform with an excitation–contraction decoupling chemical. Preliminary data using the platform to investigate the effect of the drug norepinephrine are combined with computational efforts. Finally, this platform provides a quantitative and predictive assay system that can potentially be used for comprehensive assessment of cardiac toxicity earlier in the drug discovery process.
- Published through SciTech Connect.
Lab on a chip (Print) 17 10 ISSN 1473-0197; LCAHAM AM
Fang Qian; Chao Huang; Yi-Dong Lin; Anna N. Ivanovskaya; Thomas J. O'Hara; Ross H. Booth; Cameron J. Creek; Heather A. Enright; David A. Soscia; Anna M. Belle; Ronglih Liao; Felice C. Lightstone; Kristen S. Kulp; Elizabeth K. Wheeler.
- Funding Information:
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