The unusual and dynamic character of PX-DNA [electronic resource].

Published:: Washington, D.C. : United States. Dept. of Energy. Office of Science, 2015.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description:: pages 7,201-7,206 : digital, PDF file
Additional Creators:: New York University, United States. Department of Energy. Office of Science, and United States. Department of Energy. Office of Scientific and Technical Information

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Summary:

PX-DNA is a four-stranded DNA structure that has been implicated in the recognition of homology, either continuously, or in an every-other-half-turn fashion. Some of the structural features of the molecule have been noted previously, but the structure requires further characterization. Here, we report atomic force microscopic characterization of PX molecules that contain periodically placed biotin groups, enabling the molecule to be labeled by streptavidin molecules at these sites. In comparison with conventional double stranded DNA and with antiparallel DNA double crossover molecules, it is clear that PX-DNA is a more dynamic structure. Moreover, the spacing between the nucleotide pairs along the helix axis is shorter, suggesting a mixed B/A structure. Circular dichroism spectroscopy indicates unusual features in the PX molecule that are absent in both the molecules to which it is compared.

Report Numbers:

E 1.99:1326213

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Note:

Published through SciTech Connect.
07/15/2015.
Nucleic Acids Research 43 15 ISSN 0305-1048 AM
Dong Niu; Hualin Jiang; Ruojie Sha; James W. Canary; Nadrian C. Seeman.

Funding Information:

SC0007991

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