Mechanical splitting of microtubules into protofilament bundles by surface-bound kinesin-1 [electronic resource].
- Published
- Washington, D.C. : United States. Dept. of Energy. Office of Basic Energy Sciences, 2016.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy - Physical Description
- Article numbers 39,408 : digital, PDF file
- Additional Creators
- Sandia National Laboratories, United States. Department of Energy. Office of Basic Energy Sciences, and United States. Department of Energy. Office of Scientific and Technical Information
Access Online
- Restrictions on Access
- Free-to-read Unrestricted online access
- Summary
- The fundamental biophysics of gliding microtubule (MT) motility by surface-tethered kinesin-1 motor proteins has been widely studied, as well as applied to capture and transport analytes in bioanalytical microdevices. In these systems, phenomena such as molecular wear and fracture into shorter MTs have been reported due the mechanical forces applied on the MT during transport. In the present work, we show that MTs can be split longitudinally into protofilament bundles (PFBs) by the work performed by surface-bound kinesin motors. We examine the properties of these PFBs using several techniques (e.g., fluorescence microscopy, SEM, AFM), and show that the PFBs continue to be mobile on the surface and display very high curvature compared to MT. Further, higher surface density of kinesin motors and shorter kinesin-surface tethers promote PFB formation, whereas modifying MT with GMPCPP or higher paclitaxel concentrations did not affect PFB formation.
- Report Numbers
- E 1.99:sand2017--0052j
sand2017--0052j - Subject(s)
- Note
- Published through SciTech Connect.
12/21/2016.
"sand2017--0052j"
"srep39408"
Scientific Reports 6 ISSN 2045-2322 AM
Virginia VanDelinder; Peter G. Adams; George D. Bachand. - Funding Information
- AC04-94AL85000
AC52-06NA25396
SC0001035
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